![]() A streak of dark red would have run from the cockpit to the tail. The top half of the plane would have been white and the bottom, including the belly, would have been dark blue. In 2018, Trump directed that the new jets shed the iconic Kennedy-era blue-and-white design for a white-and-navy color scheme. ![]() ![]() An Air Force review had suggested the darker colors would increase costs and delay delivery of the new jumbo jets. The choice of the plane's exterior colors follows an earlier decision by the administration to scrap a red-white-and-blue design favored by Donald Trump, Biden's immediate predecessor. They will replace the existing fleet of two aging Boeing 747-200 aircraft the president currently uses. The Air Force said late Friday that the light blue on the new model of the modified 747s that transport the president will be a little bit deeper and more modern in tone than the robin's egg blue on the versions of the aircraft currently in use.īoeing is modifying two of its 747-800 aircraft that will use the Air Force One call sign when the president is aboard. ![]() WASHINGTON - President Joe Biden is sticking with a blue-and-white color scheme for the exterior of the replacement Air Force One aircraft, the first of which is expected to be delivered in four years. ![]()
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![]() The song's chorus is often sung by audiences at Dethklok concerts, creating a powerful sense of community and shared purpose among the fans. The opening lyrics of the song, "Riding, and riding, we search across the land," were inspired by the opening lines of the classic Viking poem "Beowulf." The song's music video features the band performing the song in the middle of a freezing wasteland, surrounded by snow and wind. The artwork for the single version of The Lost Vikings features the riders on horseback in front of a massive Viking ship. The song's final verse, "All the fault of pride, the gods weep in the night," is a reference to the Norse mythology that inspired the song. The Lost Vikings is considered one of Dethklok's most popular songs, and it is often performed as an encore during their live shows. The song was included in the 2012 album "Dethalbum III," which reached number 10 on the U.S. The Lost Vikings originally premiered in the Dethklok episode of "Metalocalypse" in 2009. It is a powerful and emotive song that is both inspiring and challenging, and it speaks to the human condition in a way that few other songs do. The Lost Vikings is a song that speaks to the human heart, as it taps into our primal desire to overcome the odds and to find our place in the world. The chorus of the song, "Lost but still we ride, search until we die," speaks of the courage and the spirit of humanity that will not be defeated, no matter how harsh and unforgiving the world may be. The riders represent the determination and resilience that is required to overcome the challenges and obstacles that we face as individuals, and as societies. The lyrics of the song can be interpreted as a metaphor for the human condition, as we all, like the Lost Vikings, search for meaning, purpose, and direction in our lives. ![]() They have become lost, physically and emotionally, but they refuse to give up hope, riding on to find their destiny, even as they take on a life of wandering and searching. The riders are brave and determined, facing the harsh winter and the unknown, even when they have no map or direction. The Lost Vikings by Dethklok is a powerful and epic song of struggling against the forces of nature and time, with the lyrics speaking of a band of warriors who are searching for a battle that they feel they should have fought and won. ![]() She asks of us our story and we lie and sayīut we are far too proud and strong so we keep silent ![]() We trudge on further, eating pride and snow that's brittle Hungry and tired the frigid plain yeilds little So much time has passed since we left our land We stop, consider the land that we traveledįighting side by side to destroy our foes ![]() ![]() In addition, a franchise option is available to professional societies and other eligible organizations. The first accredited program ( IBM's) was announced in January 2006. The skill and experience requirements for both the Direct Certification and the Accredited programs are the same. In addition to certifying individuals directly, the program also provides accreditation of third-party IT architect certification programs. Under the program, practicing IT Architects can achieve the certification based on demonstrating substantial skills, experience and success in designing solutions across the whole lifecycle. The program is based on the proven best practices in the industry and sets an independent, industry-wide standard for IT Architects. The Open Group, a vendor- and technology-neutral industry consortium, offers IT Architect Certification to certify skills and experience in the IT architecture community. This program was developed by The Open Group members in response to the growing demand for experienced IT architects. The IT Architect Certification is an independent global certification program for qualifying the skills, knowledge and experience of IT, Business and Enterprise Architects. ( March 2023) ( Learn how and when to remove this template message) Please help improve it to make it understandable to non-experts, without removing the technical details. ![]() ![]() This article may be too technical for most readers to understand. ![]() ![]() All that's needed and it looked like the kickstarter that I missed was headed in that right direction. I realize there are other mail applications out there but for various reasons I don't or can't use those. There isn't a security concern here the mail is staying on the local machine for delivery. Your Mac already wakes periodically to perform tasks so sending of mail from a machine that appears asleep isn't a problem. This extension would be worth 50-100$ usd for me.Enhance Gmail with send later, tracking, templates, signatures, notes, tasks. This is Mailbutler, the email plugin which makes your inbox smarter. Mailbutler offers a multitude of useful, productivity-boosting features which you can access right in your inbox. ![]() Its features help you improve customer communication, stay on brand, optimize your email game, and much more. ![]() Send Later: Smart scheduling functionality to help you optimize your email delivery times.Recipient Optimized Scheduling: Suggests the best time for you to send an email to maximize your chances of it being opened.Email tracking: More information needed? Find out when, where, and how often your email or link was opened.Contacts: Send the perfect email for your recipient.Get essential insights into your contacts to maintain peak customer communication. Beautiful and professional email signatures with extensive creative control.Templates: Send your message in a few clicks.Craft message templates to increase productivity and efficiency. ![]() Attach tasks to your contacts and emails and keep your to-dos organized. Our dedicated customer support team responds to all queries within 24 hours.We value honesty and transparency - every user gets a 14-day free trial with no credit card required so you can see exactly what you’re in for.All your messages are secure and never accessed or read by Mailbutler.Cross-platform functionality means if other members of your team are using other email clients, Mailbutler still works perfectly.Mailbutler integrates directly and easily into your Apple Mail email client.Our extension is developed by users: we’re constantly upgrading and revamping features based on customer feedback.Productivity-lovers who want to achieve more with emails and reach Inbox Zero.Teams which need clear, easy, and productive internal communication.Businesses, marketing teams, and sales teams which prioritize direct client communication.Freelancers, independent professionals, and marketers.Share contacts, notes, tasks, signatures, templates and more with your team members.Īdd notes to your contacts and emails so you never forget any important information. Most important, Mailbutler helps you to improve your customer communication and build new prospects, clients and contacts.Integrate Mailbutler with your favorite apps, including Slack, Trello, OneNote, Todoist and more. “With Email Tracking I can focus my energy on serving clients instead of chasing emails.” Charlene Brown, Owner of Bklyn Custom Designs “Mailbutler’s support feels personal and it’s what sets them apart from so many other companies.” Craig Bowman, President of Common Ground Consulting It’s revolutionized my inbox.” Hollie Barac, Accountability and Productivity Partner “Without a doubt, I recommend Mailbutler. "Mailbutler is by far the best personal productivity app I have used. ![]() ![]() Sending memes in images is not so easy since your image is already full of other images, i.e., the game itself. Gamers often communicate with each other online while gaming either by chatting or talking. For example, a gif can only be used on WhatsApp, while an audio clip could also be used in a game or underneath a TikTok video. However, audio does have a bigger element of surprise. ![]() Some things simply cannot be replaced by audio. In the meantime, more and more apps have voice functionality.” Do you think that audio could completely replace video? Per day, around seven billion voice messages are sent. You can see this in the number of voice messages on WhatsApp. Contrary to what people tend to think, audio is gaining in popularity. But what about audio? We want to jump into that gap with Voicy. Emojis and gifs are replacing text, instagram reels are basically shortening the length of your videos. Online communications are becoming increasingly shorter and more expressive. At the moment, everything is focused on images. We want to change the way we communicate in social media. “Voicy is a huge online library where users can download and upload funny sound clips. In this instalment of Start-up of the day, Xander Kanon (27) talks about their platform and the ambition to become the biggest audio platform in the world. Their app will also be launched before the end of the year. So, they founded Voicy: an audio platform where you can share audio clips and memes. They can also be expressed via audio clips, which is something that Xander Kanon and Milan Kokir came to realize in conversation with each other. Yet such forms of expression do not always have to be images. This often involves a joke posted on the internet in the form of an image or a video. Memes are very popular among young people. Ultimate goal: That Voicy becomes part of everyday means of communication.Clients that have worked with include NBC, ESPN, PBS, The History Channel, Reader's Digest, Comcast, Nortel Networks, Bell Canada, Microsoft, Cisco Systems, ING, Western Union, Ford, GM, Jaguar, Firestone Tires, American Airlines, the US Army, the US Government and thousands more. Radio and television stations, advertising agencies, marketing executives, casting directors and voice talent agencies rely on to easily search for and hire language service providers such as translators, narrators and professional voice over talents with the assistance of their award-winning web service. ® is the online marketplace where businesses connect with voice actors and voice over talents. ![]() Similar to awards such as the Academy Awards, also known as the Oscars, honoring actors and filmmakers, the Voicey Awards recognize voice actors and voice talents for their contributions and efforts over the year in the voice over industry. The only awards show of its kind to exclusively honor voice-over professionals, the Voicey Awards features 9 categories of significance and prestige, including Best Male Voice, Best Female Voice, Best New Voice, Best Child Voice, Best Teen Voice, Best Voice Team, Best Foreign Voice, Best Personal Branding, and Lifetime Achievement. Subscribe to the podcast via iTunes or via email by visiting the VOX Talk website linked below: Stay tuned for more information and be sure to subscribe to the VOX Talk podcast to find out who the winning finalists are. Participating judges this year and their respective categories include, the first woman to announce the Academy Awards, the voice of Entertainment Tonight and The Insider, and co-author of "Voice For Hire", Randy Thomas (judging Best Male Voice) NYC casting director and author of "Acting: Make It Your Business" Paul Russell (judging Best Female Voice) super agent Billy Serow from Abrams Artists Agency in New York (judging Best New Voice) singing voice of Snow White and female movie trailer voice over pioneer, Melissa Disney (judging Best Voice Team) the original voice of Dora the Explorer, Kathleen Herles (judging Best Child Voice) British voiceover coach and talent Gary Terzza (judging Best Foreign Voice) President of Twist Image and author of the Six Pixels of Separation blog and podcast host, Mitch Joel (Best Personal Branding) and voice actress and industry blogger Bobbin Beam (judging Lifetime Achievement). "The finalists for this year's Voiceys, organized by category, and in no particular order are." Theirs are the voices of the unseen narrator, heard but not seen. Voice talents record various projects for organizations, companies, and institutions requiring voice over services including commercials for radio and television, promos, movie trailers, audio book narration, podcasts, videos, animation, videogames, telephone recordings and more. After much waiting and anticipation, finalists of the 3rd Annual Voicey Awards recognizing voice talent have been announced. ![]() ![]() In your own images you're shooting from angles that are difficult to pull off in a single frame just due to the angle between the sensor and the subject.Īrgh, I was hoping that using hardware would somehow help the software. ![]() Plenty of depth, plenty of detail, one frame. ![]() I think that stacking is useful when shooting at angles that put the depth of field at a disadvantage, but to say that it's almost essential above 1x isn't true. I have that tube as well, but haven't gotten around to using it. I will post an update about the tube when I have taken more images. Whether the effort taken to learn and use stacking software is worthwhile depends on how much you value the final images. Next we stack these in Zerene and save the results as JPGs. We always start with RAW images which we edit and convert to 16 bit TIFFS in Capture One. We end up discarding some images because it is not worth the effort required. As to the difficulties of getting stacked images without unpleasant artefacts, it can take time and practice with stacking software. We always use it for bellows and microscope photography. In my opinion, many macro shots could be improved by the extra depth of field given by stacking, and the process is almost essential with most shots having magnification beyond 1:1. I would say that the various methods we use to take the images – manual focus steps, Stackshot or Helicon FB Tube – all end with the same results. ![]() We use Zerene Stacker rather than Helicon software to stack our images. Have you found that you are getting better stacking results (i.e., less OOF artifacts) when using their hardware? I assume that you are using Helicon software for the stacking process. Something like this might just convince me to try focus stacking again. Thanks for sharing this! I'll be very interested in what else you can do with the tube and your general feelings about it as you continue to use it. In addition, of course, the tube should make it possible to cover the whole depth of subjects when a single shot could not.Īll images taken with a Canon EOS 1D Mk4 and EF 180mm macro lens. I hope that the extra depth of field provided by stacking will permit a wider range of positions from which to photograph macro subjects such as damselflies. Choosing such a viewpoint without disturbing the subject can be difficult, especially when trying to position a tripod. My normal method of making the most of the limited depth of field at macro distances is to try to get the subject parallel to the sensor in both horizontal and vertical planes. Although I only ended up with a few shots, the tube worked well enough for me to experiment further in the future. There were very few about, however, and there was a fairly strong breeze which made macro photography difficult. On my latest trip to test the Helicon FB Tube, I visited a place where I expected to see many mating pairs of damselflies at this time of year. I was satisfied with a couple of shots but the butterflies had a tendency to move their wings while I was taking the stacks, making stacking impossible. ![]() First I tried photographing butterflies at an angle (rather than parallel to the wings). When photographing simple, stationary subjects such as lichen on tree bark, the tube worked fine but the true test for me was whether I could use it to take sharp-all-over insects on photo trips. The way I use the tube is to focus on the nearest part of the subject that I want in focus and then fire the shutter until the furthest part of the subject is in focus. Once set up, the tube allows a burst of shots to be taken when the shutter is set to that mode. I did a few experiments with angled, printed paper to check the step length was about right. This distance should be small enough to ensure that the whole subject is in focus when the images are stacked, but not so small that the number of images is unnecessarily large. The tube can be preset to give a focusing increment between shots that suits the particular lens being used. I bought the tube because it is more convenient to use ‘in the field’ than our Stackshot. For taking images for stacking when using a microscope or extension bellows, we use Cognisys Stackshot equipment. I have recently been trying out a Helicon FB Tube that I bought some time ago. ![]() ![]() ![]() The first book was a Cybils nominee this year, but these opinions are mine alone and not meant to reflect the committee as a whole. We enjoyed this second installment almost as much as the first, and await the 3rd (set for release this summer!). They are excellent basketball players and identical twins, and they even excel academically! Will Owen’s jealousy get the best of him? Will Russell lose his status as the only mathlete and athlete in the school? The big news is the arrival of the Twin Towers from Minnesota, Mitch and Marcus. Russell is still trying to figure out how to best manage the Masters of the Mind team since one of their members moved, and they haven’t found a suitable replacement. Owen and Russell have made their peace with sharing the spotlight on the court. ![]() Mathlete: Double Dribble is the second in the series (with a 3rd coming out this summer!). ![]() It made for a real and funny story.Īthlete vs. Study with Quizlet and memorize flashcards containing terms like The coach wanted Russell to try out for the team because, Russell turned down Arthur's offer of money for Masters of the Mind because, Sara couldn't sell her cookies at the bake sale because and more. I loved the plotlines about sibling rivalry, family, and finding one’s place at school. Can he do both? They’ve always been able to shine in different areas, but will Russell steal Owen’s spotlight on the court? Or will sharing an interest actually make them closer? Russell also has to come to terms with being an athlete and try to balance his schoolwork and being captain of his Masters of the Mind team. When Russell makes the team, the dynamic shifts. Owen doesn’t want to get on his bad side, but he also doesn’t want his uncoordinated brother to embarrass him. When a new basketball coach comes to their middle school, he encourages Russell to try out for the team, thinking his height will be an advantage.īoth Russ and Owen are floored by this news, but the tough coach doesn’t really present it as an option, but as a requirement. Owen is strong and stocky and Russell is tall and gangly. In addition to their different strengths, the fraternal twins look completely different as well. This story is told in alternating chapters from the point of view of twin brothers Owen, the athlete, and Russell, the mathlete. My 4th grade son is neither a mathlete nor an athlete, but is closer to the former than the latter. My son and I really enjoyed reading Athlete vs. ![]() ![]() Before you go balayage, make sure you choose the tones that are the most representative for you. Regardless if you go for earth tones or experiment with pastels or bright hues, this technique will make your hair glow beautifully. In conclusion, there are more than enough balayage hair color ideas to pick from. Blue Steel Semi Permanent Cream Hair Color Save. You simply can’t go wrong by choosing the subtle changes brought about by these hairstyles. Sally Beauty has the largest selection of salon-professional Silver & Gray Hair Color. Pearl Blonde, Silver Blonde, Blue Based Ash Blonde Toners. ![]() The balayage hair coloring technique is really a one-size-fits-all. Silver blue balayage Blue Balayage, Hairstyle Zodiac, Silver Blue Hair, Easy Hairstyles. This color technique plays off your tresses’ natural movement and does not leave any signs of delineation that comes from using hair coloring tools. Match it with a long bob and you are sure to have a perfect look! Balayage color is painted on with soft bush strokes across the surface of the hair, resulting in natural-looking swathes of color, from thick to thin. silverhaircolor silverhair silvertitaniumhair silverblondehair. The best way to test Garnier hair color is to try before you buy Trying on any shade is now as easy as using a filter, thanks to our Virtual Try-On. We have silver hair color ideas for you to choose from. The copper balayage is ideal for medium length to long hair, as the highlights will pop out more on longer strands of hair. Then consider this silver titanium blonde hair created by hair artist-colorist Kim Wasabi (kimwasabi). I always say that it is not "grey hair" which makes you look old, it's your full style which counts.This classy warm color is both intense and versatile. ‘In terms of styling – wear colour, make-up or anything you like to look your best. Cant go wrong with a high contrast silver balayage for brunettes. The transition takes many months and there will be moments when you feel tempted to go back to colour. Being sure that you want to go grey is paramount. ‘The starting point is making the decision. She was so smart and looked incredibly confident, I loved that.’ What tips would you offer women looking to start the growing out process? The denim hair color effect really relies on texture, by having highlights and lowlights. ‘One of my main inspirations was Meryl Streep's character in The Devil Wears Prada. I found it impossible to dedicate 3 hours every two weeks to colour my hair.’ So, inspired by London's free-spirited approach to fashion and beauty, Silvina took on the decision to let her natural hair colour grow out. ‘I had just moved to London and was struggling with managing my time. ‘I always questioned why white-haired men were considered silver foxes, whereas grey-haired women were seen as old,’ says Silvina. The bleach was applied in a balayage style that blended with my natural color. Spiky & Faded Mens Grey Hair Dye Textured Crop Ash Grey Hair Men Slicked Back Undercut Brushed Up And Messy Short Crop Top Fade Silver Blue Hair Dark. ![]() After moving to London in 2015, Silvina decided to embrace her natural grey hair – which in turn, set about a whole career change.īelow, she tells Women's Health the lessons she's learnt along the way, and what she wishes she knew about confidence growing up… What inspired you to decide to embrace your natural hair colour? That took a professional stylist almost 6 hours and cost around 250 before tip. ![]() So why is there still such a stigma surrounding one of the most natural colours in the world?Īrgentinian model Silvina Neder is looking to change the narrative around grey hair. From brunette balayage to copper hair, it's often said that anything goes. If you are looking for a darker shade, dye your hair midnight blue, as you see here on singer Megan Thee Stallion. When it comes to hair colour – it can seem like the options are endless. ![]() ![]() ![]() ![]() To learn more about how and for what purposes Amazon uses personal information (such as Amazon Store order history), please visit our Privacy Notice. You can change your choices at any time by visiting Cookie Preferences, as described in the Cookie Notice. Click ‘Customise Cookies’ to decline these cookies, make more detailed choices, or learn more. Third parties use cookies for their purposes of displaying and measuring personalised ads, generating audience insights, and developing and improving products. This includes using first- and third-party cookies, which store or access standard device information such as a unique identifier. If you agree, we’ll also use cookies to complement your shopping experience across the Amazon stores as described in our Cookie Notice. We also use these cookies to understand how customers use our services (for example, by measuring site visits) so we can make improvements. Grief is one thing that can’t be avoided for long.We use cookies and similar tools that are necessary to enable you to make purchases, to enhance your shopping experiences and to provide our services, as detailed in our Cookie Notice. She’s the farthest thing from being fine). How is Charlene supposed to grieve when she’s also fulfilling her obligation to carry on her brother’s design business, care for her aging mother, Lorenna, and reconcile with Ayla, who she’s been avoiding? No big deal. Charlene’s husband, Raul, has moved him and their two young kids to live with his sister to allow Charlene the time and space to grieve. Arthur and the Invisibles 2006, 1h 34min - Kids From the creative mind of talented filmmaker Luc Besson ('La Femme Nikita', 'The Fifth Element'), comes a larger-than-life, family adventure about a boy who, after his grandfather disappears, sets out to save his family home from emerging real estate developers. But she has to face her grief, which has resulted in marital problems. Charlene’s life feels completely wrecked as she attempts to salvage her fledgling acting and screenwriting career. Neither Charlene, Ayla, her pregnant sister-in-law, or mother Lorenna could have seen this tragedy coming. It’s been 9 months now since Dominick’s death and Charlene still hasn’t been able to fully process this loss. From the creative mind of talented filmmaker Luc Besson ('La Femme Nikita', 'The Fifth Element'), comes a larger-than-life, family adventure about a boy who, after his grandfather disappears, sets out to save his family home from emerging real estate developers. LAST CHANCE CHARLENE tells the story of Charlene, a writer/actress trying to put her life back together after losing her brother, Dominick, to suicide. A journey that takes you through every human emotion, iMordecai teaches you that it’s never too late for inspiration. Ultimately, through letting go, he begins to heal the things he cannot fix and turn the pain of his past into something beautiful. He becomes open to new experiences and new perspectives. Obtenido de youtube/watch vwupLvFhc3Hs&t35s&abchannel Bioqu. ![]() Webconferencia 2 Tarea 3 Enzimología y Bioenergética. Unidad 1 Tarea 2 Técnicas de conteo y Teoría de la. Mordecai becomes friends with the “Einstein’s,” led by Nina (Azia Dinea Hale), who teach him to use this strange device with no buttons. Tratado de fisiologia Medica (Arthur Clifton Guyton John E. This act sets off a series of unexpected events and magically changes both Mordecai and the people closest to him. When Arthurs grandfather disappeared four years ago, leaving behind nothing but. For Mordecai, this moment happens when his son, Marvin, replaces his ancient phone with a brand-new iPhone. From visionary director, Luc Besson, comes this spectacular family adventure mix of live-action and state-of-the-art CG animation with an all-star voice cast that includes Freddie Highmore, Robert De Niro, Madonna, Snoop Dogg and David Bowie. (Sean Astin) Sometimes, something small and unexpected can change your whole world. But this is a story about the things he cannot fix: Getting older, the Alzheimer’s diagnosis of his wife Fela (Carol Kane), and his relationship with his son Marvin. It’s why he worked his entire life as a plumber and a painter. It’s why his phone is 20 years old and held together with duct tape and tinfoil. Mordecai (Judd Hirsch) likes to fix things. ![]() ![]() ![]() The location of FileMerge is determined through a symbolic link in the PATH environment. Some wrapper scripts are thus required to call FileMerge from Subversion.īash scripts for this task can be checked-out from this repository or downloaded as an archive.įour scripts are provided: fmdiff, fmdiff3, fmresolve and fmmerge. it forks and does not block) and it expects different arguments. It can be opened from the command line with the opendiff command, but its interface differs from that of diff and diff3. Worthless to find interline changes in long linesĪpple's Developer Tools for Mac OS X include FileMerge, a graphical tool to compare and merge files.įileMerge can be much handier to use unfortunately, it doesn't integrate with Subversion straightforwardly.They do have some limitations though, including: The built-in diff and diff3 tools (from diffutils) go a long way to provide this functionality for text files on the command line. Subversion principally uses the copy-modify-merge versioning model to allow concurrent collaboration on the same set of files.įor this to work well, it is crucial to have good tools to view and merge the differences between files. The official website (and official version) can be found at: These scripts were created by Bruno De Fraine. doi:10.1002/cncy.Using FileMerge as a diff command for Subversion Multiplatform comparison of molecular oncology tests performed on cytology specimens and formalin-fixed, paraffin-embedded tissue. Adequacy of samples obtained by EBUS TBNA for molecular analysis in patients with non-small cell lung cancer. Labarca G, Folch E, Jantz M, Mehta HJ, Majid A, Fernandez-Bussy S. Recommendations for the use of next-generation sequencing (NGS) for patients with metastatic cancers: a report from the ESMO Precision Medicine Working Group. Molecular testing guideline for the selection of lung cancer patients for treatment with targeted tyrosine kinase inhibitors: American Society of Clinical Oncology endorsement summary of the College of American Pathologists/International Association for the Study of Lung Cancer/Association for Molecular Pathology clinical practice guideline update. Endobronchial ultrasound-guided transbronchial needle aspiration cytology: a state of the art review. ![]() Cancer Cytopathology published by Wiley Periodicals LLC on behalf of American Cancer Society.Ĭameron SEH, Andrade RS, Pambuccian SE. ![]() TruSight Oncology 500 (TSO500) cytology endobronchial ultrasound-guided transbronchial needle aspiration (EBUS TBNA) lung cancer molecular diagnostics. Microscopy of Diff-Quik smears can triage samples for comprehensive panel sequencing, which highlights smears as an excellent alternative to traditional testing with cell blocks. Smears were scored for tumor mutation burden. Tier 1/2 mutations were discovered in two cases where standard-of-care specimens were inadequate for sequencing. All standard-of-care mutations were detected in replicate smears from individual patients, regardless of malignant cell content. Low-malignant-cell content (<25%) and smear area (<15%) were the main reasons for failure. Fifty DNA samples (89%) with varying malignant yields were successfully sequenced. An algorithm was developed that predicted a >50-ng DNA yield of a smear with an area under the curve of 0.86. DNA from 56 smears from 27 patients was sequenced with the TruSight Oncology 500 assay (Illumina).Įach microscopy parameter had a significant effect on the DNA yield. One hundred eighty-five smears were evaluated by microscopy to estimate malignant cell percentage and abundance and to calculate smear size and were subjected to DNA extraction. All patients had air-dried, Diff-Quik cytology smears and formalin-fixed, paraffin-embedded cell blocks collected for cytopathology and molecular testing. Studies are needed to demonstrate their great potential, in particular for the prediction of malignant cell DNA content and for utility in molecular diagnostics using large gene panels.Ī prospective study was performed on samples from 66 patients with malignant lymph nodes who underwent EBUS TBNA. Cytology smears are commonly collected during endobronchial ultrasound-guided transbronchial needle aspiration (EBUS TBNA) procedures but are rarely used for molecular testing. ![]() |